Under supervision of the Principal Investigator, Neuroscience this position is responsible for maintenance of the transgenic colonies including breeding, timed breeding, collection of samples for genotyping, weaning, aging and sexing of pups, and retiring of mouse colonies. This position is also responsible for mouse behavioral battery tests and their analyses.
Creates breeding pairs or trios and plans breeding strategies to meet the demands of investigators.
Handles live mice to perform all colony management duties, including but not limited to the following:
Identification and aging of new litters;
Handling pregnant and nursing animals;
Handling, sexing, and identification of pups;
Euthanizing animals; and
Printing cage cards.
Sets up breeding cages as necessary.
Completes genotyping process including tag and snip, and documentation of genotypes and strain histories.
Maintains records of breeding, weaning, and genotype results of the colony.
Conducts mouse behavioral battery testing.
Performs basic molecular/cellular experiments, including immunocytochemistry, western blotting and RT-qPCR.
Communicates regularly with veterinary staff and with other members of the technical staff.
Follows all SOP’s relating to the care, breeding, and use of the animals.
Assists other vivarium personnel with laboratory duties as needed.
Perform other duties as required by their supervisor.
Bachelor’s degree in biology, molecular biology or related discipline and a minimum of 2 years of experience of handling rodents (mice or rats) for behavioral testing.
Prior experience working in laboratory animal sciences with rodent behavioral testing.
Experience in the management and breeding of traditional and/or transgenic rodents.
Demonstrated ability to collect tissue sampling for rodent genotyping (tail snip).
Demonstrated ability of conducting basic molecular and cellular experiments on the animal tissues, including immunocytochemistry, western blotting and RT-qPCR.
Demonstrated ability to recognize and report common health problems in laboratory rodents.
Must have excellent organizational and planning skills and ability to use computerized databases for scheduling procedures, time management, data entry, graph preparation, etc.
Demonstrated ability to coordinate and maintain breeding records for laboratory animals, both electronic and hard copy.
Demonstrated ability to work with investigators to understand and coordinate their research projects with animal needs
Must have excellent written and verbal communication skills.
Ability to work in a team environment.
Ability to work overtime when necessary and rotating weekends and holidays.
Certification at the LAT level or higher.
Demonstrated ability to assess, monitor, and treat laboratory rodents under supervision of the veterinary staff (veterinarian or veterinary technician).